Method Validation for Pesticide Residues on Rice Grain in Aceh Besar District, Indonesia Using Gas Chromatography-Electron Capture Detector (GC-ECD)

Authors

  • Agus Winarsih Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Rinaldi Idroes Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Utari Zulfiani Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Department of Chemistry, Faculty of Science and Data Analytics, Institut Teknologi Sepuluh Nopember, Sukolilo, Surabaya 60111, Indonesia
  • Muhammad Yusuf Department of Pharmacy, STIKES Assyifa Aceh, Banda Aceh 23242, Indonesia
  • Mahmudi Mahmudi Department of Pharmacy, STIKES Assyifa Aceh, Banda Aceh 23242, Indonesia
  • Saiful Saiful Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Sunarti Abd Rahman Faculty of Chemical & Process Engineering Technology, Universiti Malaysia Pahang, Lebuhraya Persiaran Tun Khalil Yaakob, 26300 Gambang, Kuantan, Pahang, Malaysia

DOI:

https://doi.org/10.60084/ljes.v1i1.37

Keywords:

Bifenthrin, Dichlorvos, Dimethoate, GC-ECD, Rice, λ-Cyhalothrin

Abstract

Analysis of pesticide residues in rice in Aceh Besar District using the Gas Chromatography – Electron Capture Detector (GC-ECD) method has been carried out. This study aims to validate the analytical method and determine the pesticide residue levels of Dichlorvos, Dimethoate, Bifenthrin, and λ-Cyhalothrin in rice samples. Rice samples in branded rice were taken from the Districts of Want Jaya, Indrapuri, Darussalam, Suka Makmur, Simpang Tiga, Kuta Baro, and ground using a grinder. The powder sample was extracted by the QuEChERS method and analyzed by GC-ECD. The results of the linearity test have met the requirements with the coefficient of determination (R2), which is an average of 0.98. The LOD values ranged from 0.013 to 0.017 mg/kg, while the LOQ ranged from 0.022 to 0.079 mg/kg. The results of precision and reproducibility (% RSD, n = 6) show the values of 0.56 - 1.26% and 1.14 - 2.19%, respectively, and the accuracy value (%Recovery) shows the results of 99.71 - 101.84%, with an RSD value of 2.42 - 3.59%, meet the requirement of 20%. The results of the analysis of the sample showed that sample A had a large %Recovery value in the Dichlorvos analyte, namely 139.10%, with the calculation that the Dichlorvos analyte contained 0.0206 mg/Kg. This value has not passed the MLR set by the European Food Safety Authority, which is 0.2 mg/Kg. In the other rice samples, no pesticide residue analytes were detected. The calculation of %Recovery of each analyte in the spiked sample ranged from 80-101%, which indicated that the pesticide residue analysis carried out had good accuracy, namely the requirement of 70-120%.

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Published

2023-06-30

How to Cite

Winarsih, A., Idroes, R., Zulfiani, U., Yusuf, M., Mahmudi, M., Saiful, S., & Rahman, S. A. (2023). Method Validation for Pesticide Residues on Rice Grain in Aceh Besar District, Indonesia Using Gas Chromatography-Electron Capture Detector (GC-ECD). Leuser Journal of Environmental Studies, 1(1), 18–24. https://doi.org/10.60084/ljes.v1i1.37

Issue

Vol. 1 No. 1 (2023): July 2023

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Articles

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Copyright (c) 2023 Agus Winarsih, Rinaldi Idroes, Utari Zulfiani, Muhammad Yusuf , Mahmudi Mahmudi, Saiful Saiful, Sunarti Abd Rahman

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