Sustainable Biohydrogen Production from Palm Oil Mill Effluent: Effect of Hydraulic Retention Time in a Hybrid Anaerobic System

Authors

  • Adrianto Ahmad Department of Chemical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia
  • Evelyn Evelyn Department of Chemical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia
  • David Andrio Department of Chemical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia
  • Dini Avriliani Department of Chemical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia
  • M. Dalil Department of Mechanical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia
  • Amir Hamzah Department of Electrical Engineering, Universitas Riau, Pekanbaru 28293, Indonesia

DOI:

https://doi.org/10.60084/ljes.v4i1.379

Keywords:

Anaerobic hybrid bioreactor, Climate change, Renewable energy, Utilizing POME

Abstract

Currently, the world is facing two crises: a shortage of fossil fuels and global climate change. Climate change is linked to increased environmental damage from fossil fuel use and the effects of greenhouse gases. Therefore, it is important to achieve breakthroughs to develop alternative energy sources that can replace fossil fuels. One of these is biohydrogen, which plays an important role in future energy because it is environmentally friendly, renewable, and sustainable. In addition, Indonesia is the world's largest producer of palm oil, which naturally generates liquid waste. Using palm oil mill liquid waste to produce biohydrogen via an anaerobic hybrid bioreactor during the acidogenesis phase is the best solution to address environmental impacts while simultaneously providing a clean energy source. This research aims to produce biohydrogen from palm oil mill liquid waste. This was done using an anaerobic hybrid bioreactor during the acidogenesis phase, with hydraulic retention times of 6, 12, and 18 hours. The research results show that the best hydraulic retention time is 18 hours, with a VSS removal efficiency of 98% and biogas production of 5.0 L/day, yielding 64% biohydrogen.

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References

  1. Ahmed, S. F., Mofijur, M., Parisa, T. A., Islam, N., Kusumo, F., Inayat, A., Le, V. G., Badruddin, I. A., Khan, T. M. Y., and Ong, H. C. (2022). Progress and Challenges of Contaminate Removal from Wastewater Using Microalgae Biomass, Chemosphere, Vol. 286, 131656. doi:10.1016/j.chemosphere.2021.131656.
  2. Mahlia, T. M. I., Syazmi, Z. A. H. S., Mofijur, M., Abas, A. E. P., Bilad, M. R., Ong, H. C., and Silitonga, A. S. (2020). Patent Landscape Review on Biodiesel Production: Technology Updates, Renewable and Sustainable Energy Reviews, Vol. 118, 109526. doi:10.1016/j.rser.2019.109526.
  3. Mahmudul, H. M., Rasul, M. G., Akbar, D., Narayanan, R., and Mofijur, M. (2021). A Comprehensive Review of the Recent Development and Challenges of a Solar-Assisted Biodigester System, Science of The Total Environment, Vol. 753, 141920. doi:10.1016/j.scitotenv.2020.141920.
  4. Mofijur, M., Siddiki, S. Y. A., Shuvho, M. B. A., Djavanroodi, F., Fattah, I. M. R., Ong, H. C., Chowdhury, M. A., and Mahlia, T. M. I. (2021). Effect of Nanocatalysts on the Transesterification Reaction of First, Second and Third Generation Biodiesel Sources- A Mini-Review, Chemosphere, Vol. 270, 128642. doi:10.1016/j.chemosphere.2020.128642.
  5. Wang, J., and Yin, Y. (2018). Fermentative Hydrogen Production Using Pretreated Microalgal Biomass as Feedstock, Microbial Cell Factories, Vol. 17, No. 1, 22. doi:10.1186/s12934-018-0871-5.
  6. Bolatkhan, K., Kossalbayev, B. D., Zayadan, B. K., Tomo, T., Veziroglu, T. N., and Allakhverdiev, S. I. (2019). Hydrogen Production from Phototrophic Microorganisms: Reality and Perspectives, International Journal of Hydrogen Energy, Vol. 44, No. 12, 5799–5811. doi:10.1016/j.ijhydene.2019.01.092.
  7. Gielen, D. (2019). Hydrogen: A Renewable Energy Perspective, IRENA, Abu Dhabi.
  8. Nagarajan, D., Dong, C., Chen, C., Lee, D., and Chang, J. (2021). Biohydrogen Production from Microalgae—Major Bottlenecks AnNagarajan, D., Dong, C., Chen, C., Lee, D., & Chang, J. (2021). Biohydrogen Production from Microalgae—Major Bottlenecks and Future Research Perspectives. Biotechnology Journal, 16(5), 2000124. Ht, Biotechnology Journal, Vol. 16, No. 5, 2000124. doi:10.1002/biot.202000124.
  9. Das, D., Khanna, N., and Dasgupta, C. N. (2019). Biohydrogen Production: Fundamentals and Technology Advances, CRC Press, Boca Raton.
  10. Dawood, F., Anda, M., and Shafiullah, G. M. (2020). Hydrogen Production for Energy: An Overview, International Journal of Hydrogen Energy, Vol. 45, No. 7, 3847–3869. doi:10.1016/j.ijhydene.2019.12.059.
  11. Nagarajan, D., Lee, D.-J., Kondo, A., and Chang, J.-S. (2017). Recent Insights into Biohydrogen Production by Microalgae – From Biophotolysis to Dark Fermentation, Bioresource Technology, Vol. 227, 373–387. doi:10.1016/j.biortech.2016.12.104.
  12. Abdalla, A. M., Hossain, S., Nisfindy, O. B., Azad, A. T., Dawood, M., and Azad, A. K. (2018). Hydrogen Production, Storage, Transportation and Key Challenges with Applications: A Review, Energy Conversion and Management, Vol. 165, 602–627. doi:10.1016/j.enconman.2018.03.088.
  13. Dewan Energi Nasional. (2023). Indonesia Energy Outlook, Secretariat General of the National Energy Council, Jakarta.
  14. Yusnitati, Anindita, H. N., Adha, A., Septriana, D., Priambodo, T. B., Hastuti, Z. D., Santoso, E., Wulandari, W., Zuldian, P., Primeia, S., Baruji, T., Yurismono, H., Prasetyo, D. H., Murti, S. D. S., Senda, S. P., and Saputra, H. (2025). Biohydrogen Production from Palm Oil Mill Effluent (POME) in Indonesia: Potential, Challenges, and Prospects, International Journal of Hydrogen Energy, Vol. 138, 1315–1335. doi:10.1016/j.ijhydene.2024.11.277.
  15. Ismail, I., Hassan, M. A., Abdul Rahman, N. A., and Soon, C. S. (2010). Thermophilic Biohydrogen Production from Palm Oil Mill Effluent (POME) Using Suspended Mixed Culture, Biomass and Bioenergy, Vol. 34, No. 1, 42–47. doi:10.1016/j.biombioe.2009.09.009.
  16. Ahmad. (2019). Wastewater Treatment Method with High Organic Load Using Two-Phase Anaerobic Partition Bioreactor.
  17. Antonopoulou, G., Gavala, H. N., Skiadas, I. V, Angelopoulos, K., and Lyberatos, G. (2008). Biofuels Generation from Sweet Sorghum: Fermentative Hydrogen Production and Anaerobic Digestion of the Remaining Biomass, Bioresource Technology, Vol. 99, No. 1, 110–119. doi:10.1016/j.biortech.2006.11.048.
  18. Zusri, A. S., Sidabutar, R., Irvan, I., Trisakti, B., Michael, M., Sarah, M., Tambun, R., and Daimon, H. (2025). Bioreactor Design for Acidogenesis Process of Palm Oil Mill Effluent, Journal of Ecological Engineering, Vol. 26, No. 8, 16–26. doi:10.12911/22998993/203520.
  19. Ahmad, A., Bahruddin, Andrio, D., and Hamzah, A. (2019). The Performance of a Pilot-Scale Anaerobic Hybrid Bioreactor on Palm Oil Mill Effluent Treatment, Reaktor, Vol. 19, No. 3, 111–116.
  20. APHA, AWWA, and WCPF. (1992). Standard Methods for the Examination of Waters and Wastewater (20th Editi.), American Public Health Association, Washington DC.
  21. Setiadi, T., Ahmad, A., and Wenten, I. G. (2000). Pemakaian Bioreaktor Membran Untuk Pengolahan Limbah Cair Industri, Proceedings of 1st National Seminar on Chemical Process Technology II, Jakarta.
  22. Wong, Y.-S., Teng, T. T., Ong, S.-A., Norhashimah, M., Rafatullah, M., and Lee, H.-C. (2013). Anaerobic Acidogenesis Biodegradation of Palm Oil Mill Effluent Using Suspended Closed Anaerobic Bioreactor (SCABR) at Mesophilic Temperature, Procedia Environmental Sciences, Vol. 18, 433–441.
  23. Faisal, M., Machdar, I., Gani, A., and Daimon, H. (2016). The Combination of Air Flotation and a Membrane Bioreactor for the Treatment of Palm Oil Mill Effluent, International Journal of Technology, Vol. 7, No. 5, 767. doi:10.14716/ijtech.v7i5.3163.
  24. Maulina, S., Matseh, I., Trisakti, B., and Daimon, H. (2018). Development of Anaerobic Digestion of Palm Oil Mill Effluent with Heated Recycle Sludge, RASAYAN Journal of Chemistry, Vol. 11, No. 3, 1151–1158.
  25. Jijai, S., Siripatana, C., O-Thong, S., and Ismail, N. (2016). Kinetic Models for Prediction of Cod Effluent from Upflow Anaerobic Sludge Blanket (Uasb) Reactor for Cannery Seafood Wastewater Treatment, Jurnal Teknologi, Vol. 78, Nos. 5–6. doi:10.11113/jt.v78.8644.
  26. Anggamulia, M. I., Syafila, M., Handajani, M., and Gumilar, A. (2020). The Potential Bio-Conversion of Palm Oil Mill Effluent (POME) as Bioethanol by Steady-State Anaerobic Processes, E3S Web of Conferences, Vol. 148, 02001. doi:10.1051/e3sconf/202014802001.
  27. Ng, W. J., Wong, K. K., and Chin, K. K. (1985). Two-Phase Anaerobic Treatment Kinetics of Palm Oil Waste, Water Research, Vol. 19, No. 5, 667–669.
  28. Nurkholis, Sarto, and Hidayat, M. (2016). Effect of Hydraulic Retention Time on Biohydrogen Production from Melon Fruit Waste (Cucumis Melo L.) Using a Continuous Pipeline Flow Reactor, Chemical Engineering Innovation, Vol. 1, No. 2. doi:10.31942/inteka.v1i2.1652.
  29. Pavlostathis, S. G., and Giraldo‐Gomez, E. (1991). Kinetics of Anaerobic Treatment: A Critical Review, Critical Reviews in Environmental Control, Vol. 21, Nos. 5–6, 411–490. doi:10.1080/10643389109388424.

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Published

2026-04-30

How to Cite

Ahmad, A., Evelyn, E., Andrio, D., Avriliani, D., Dalil, M., & Hamzah, A. (2026). Sustainable Biohydrogen Production from Palm Oil Mill Effluent: Effect of Hydraulic Retention Time in a Hybrid Anaerobic System. Leuser Journal of Environmental Studies, 4(1), 69–78. https://doi.org/10.60084/ljes.v4i1.379

Issue

Vol. 4 No. 1 (2026): April 2026

Section

Articles

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Copyright (c) 2026 Adrianto Ahmad, Evelyn Evelyn, David Andrio, Dini Avriliani, M. Dalil, Amir Hamzah

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.