Antibacterial Activity of n-Hexane Dragon’s Blood Resin Extract (Daemonorops draco wild Blume) from Bener Meriah, Aceh Province, Indonesia

Authors

  • Khairan Khairan Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Herbal Medicinal Research Centre, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Atsiri Research Centre, PUI-PT-Nilam, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Musfira Arini Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Rinaldi Idroes Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Herbal Medicinal Research Centre, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia; Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Khalijah Awang Department of Chemistry, Faculty of Science, University of Malaya, Malaysia
  • Claus Jacob School of Pharmacy, Universitaet des Saarlandes, Saarbruecken, Germany

DOI:

https://doi.org/10.60084/mp.v1i1.29

Keywords:

Dragon’s blood resin, Bener Meriah, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Candida albicans 10231

Abstract

The dragon’s blood resin (Daemonorops draco wild Blume) has been used in folk medicine for pharmacological activities such as antimicrobial, antivirus, anti-inflammation, gastrointestinal disorders, blood circulation dysfunctions, antitumor, and cancer. This study was designated to evaluate the antibacterial activity of n-Hexane dragon’s blood resin extract against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans 10231. The other purpose of this study was to determine the secondary metabolites compound of n-Hexane dragon’s blood resin extract. The antimicrobial activities of the n-Hexane dragon’s blood resin extract was determined using well diffusion method and the results showed that the extract at concentration of 15% exhibited antimicrobial activities against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Candida albicans 10231 with the diameter inhibition of 13.20 mm; 21.3 mm; and 13.0 mm respectively. The phytochemicals screening showed that the extract contains secondary metabolites in the form of flavonoids. The GC-MS analysis showed that n-Hexane dragon’s blood resin extract contains 48 chemicals compounds, and the compound at RT 26 was indicated a Drachorhodin compound (C17H 18O3) with the mass ration of m/z was 270 g/mol. Overall, the n-Hexane dragon’s blood resin extract be a good choice for antimicrobial agent against bacteria and fungi.

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References

  1. Gupta, D., Bleakley, B., Gupta, R. K. (2008). Dragon’s blood: Botany, chemistry and therapeutic uses, Journal of Ethnopharmacology, Vol. 115, No. 3, 361–380. doi:10.1016/j.jep.2007.10.018
  2. Namjoyan, F., Kiashi, F., Moosavi, Z. B., Saffari, F., Makhmalzadeh, B. S. (2016). Efficacy of Dragon’s blood cream on wound healing: A randomized, double-blind, placebo-controlled clinical trial, Journal of Traditional and Complementary Medicine, Vol. 6, No. 1, 37–40. doi:10.1016/j.jtcme.2014.11.029
  3. Mahmood, S., Fatima, T., Zulfaqar, H., Saher, R., Rafiq, M., Rehman, A., Dai, R., Ashfaq, M., Hasan, M. (2017). Meta-analysis of dragon’s blood resin extract as radio-protective agent, Journal of Coastal Life Medicine, 409–416. doi:10.12980/jclm.5.2017J7-75
  4. Purwanti, S. (2017). Aktivitas Antioksidan, Antibakteri, dan Antibiofilm Resin Jernang (Daemonorops draco)., Bogor Agricultural University (IPB)
  5. Nisah, K., Fahrina, A., Rizki, D. R., Puspita, K. (2023). Optimization of Starch—κ-Carrageenan Hybrid Film as Drug Delivery System using Response Surface Method, Heca Journal of Applied Sciences, Vol. 1, No. 1, 19–23. doi:https://doi.org/10.60084/hjas.v1i1.10
  6. Toriq, U. (2013). Senyawa kimia penciri jernang untuk pembaruan parameter standar nasional Indonesia
  7. Eissa Mohammed, Y. H. (2016). In-vitro Anti-Cancer Activity of Extracts Dracaen Cinnabari Balf. F Resin from Socotra Island in Yemen Republic, Biochemistry & Analytical Biochemistry, Vol. 5, No. 3. doi:10.4172/2161-1009.1000296
  8. Kartono Waluyo, T., Pasaribu, G. (2015). Aktivitas Antijamur, Antibakteri dan Penyembuhan Luka Ekstrak Resin Jernang, Jurnal Penelitian Hasil Hutan, Vol. 33, No. 4, 377–385. doi:10.20886/jphh.2015.33.4.377-385
  9. K Waluyo, T., Pasaribu, G. (2013). Aktifitas Antioksidan dan Antikoagulasi Resin Jernang, Jurnal Penelitian Hasil Hutan, Vol. 31, No. 4, 306–315. doi:10.20886/jphh.2013.31.4.306-315
  10. Departemen Kesehatan RI. (2000). Farmakope Indonesia Edisi IV, Depkes RI, Jakarta
  11. Organization, W. H. (2003). Manual for the laboratory identification and antimicrobial susceptibility testing of bacterial pathogens of public health importance in the developing world: Haemophilus influenzae, Neisseria meningitidis, Streptococcus pneumoniae, Neisseria gonorrhoea, SWorld Health Organization
  12. Harborne, J. B. (1987). Phytochemical Method: A Guide To Modern Techniquws of Plant Analysis, Oleh Kosasih Padmawinata Dan Iwang Sudiro, ITB, Bandung
  13. Khairan, K., Hasan, M., Idroes, R., Diah, M. (2023). Fabrication and Evaluation of Polyvinyl Alcohol/Corn Starch/Patchouli Oil Hydrogel Films Loaded with Silver Nanoparticles Biosynthesized in Pogostemon cablin Benth Leaves’ Extract, Molecules, Vol. 28, No. 5, 2020. doi:10.3390/molecules28052020
  14. Ismalita, Khairan, K., Rahmi, Muhammad, S., Indra, I. (2022). Green synthesis of sulfur nanoparticles using Allium sativum: Its effects on the growth of Pogostemon cablin Benth. and the chemical characterization of the patchouli oil after being harvested, Nanotechnology for Environmental Engineering, Vol. 7, No. 2, 359–375. doi:10.1007/s41204-022-00217-5
  15. Minarni, Muhaimin, Demando, G. (2020). Antibacterial Activity of Ethanol Extract of Dragon’s Blood (Daemonorops didymophylla) Against Salmonella typhi and Streptococcus mutants, Jurnal Akta Kimia Indonesia, Vol. 13, No. 2. doi:http://dx.doi.org/10.20956/ica.v13i2.10506
  16. Pelczar, M. J., Chan, E. C. S. (1988). Dasar-dasar mikrobiologi jilid 2, Penerbit UI, Jakarta
  17. Gupta, D., Gupta, R. K. (2011). Bioprotective properties of Dragon’s blood resin: In vitro evaluation of antioxidant activity and antimicrobial activity, BMC Complementary and Alternative Medicine, Vol. 11, No. 1, 13. doi:10.1186/1472-6882-11-13
  18. Shi, J., Hu, R., Lu, Y., Sun, C., Wu, T. (2009). Single-step purification of dracorhodin from dragon’s blood resin of Daemonorops draco using high-speed counter-current chromatography combined with pH modulation, Journal of Separation Science, Vol. 32, Nos. 23–24, 4040–4047. doi:10.1002/jssc.200900392
  19. Silverstein, R. M., Webster, F. X., Kiemle, D. J. (2005). Spectrometric Identification of Organic Compounds 7th Editio, John Wiley & Son Inc, New York
  20. Brockmann, H. (1943). Constitution of dracorhodin, a new pigment from" dragon’s blood", Berl Dtsch Chem Ges, Vol. 76, 751–763
  21. Xia, M., Wang, D., Wang, M., Tashiro, S., Onodera, S., Minami, M., Ikejima, T. (2004). Dracorhodin Perchlorate Induces Apoptosis via Activation of Caspases and Generation of Reactive Oxygen Species, Journal of Pharmacological Sciences, Vol. 95, No. 2, 273–283. doi:10.1254/jphs.FPJ03102X
  22. Baumer, U., Dietemann, P. (2010). Identification and differentiation of dragon’s blood in works of art using gas chromatography/mass spectrometry, Analytical and Bioanalytical Chemistry, Vol. 397, 1363–1376
  23. Melo, M. J., Sousa, M., Parola, A. J., de Melo, J. S. S., Catarino, F., Marçalo, J., Pina, F. (2007). Identification of 7, 4′‐Dihydroxy‐5‐methoxyflavylium in “Dragon’s Blood”: To Be or Not To Be an Anthocyanin, Chemistry–A European Journal, Vol. 13, No. 5, 1417–1422
  24. Edwards, H. G. M., de Oliveira, L. F. C., Prendergast, H. D. V. (2004). Raman spectroscopic analysis of dragon’s blood resins—basis for distinguishing between Dracaena (Convallariaceae), Daemonorops (Palmae) and Croton (Euphorbiaceae), The Analyst, Vol. 129, No. 2, 134–138. doi:10.1039/B311072A

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Published

2023-06-17

How to Cite

Khairan, K., Arini, M., Idroes, R., Awang, K., & Jacob, C. (2023). Antibacterial Activity of n-Hexane Dragon’s Blood Resin Extract (Daemonorops draco wild Blume) from Bener Meriah, Aceh Province, Indonesia. Malacca Pharmaceutics, 1(1), 22–29. https://doi.org/10.60084/mp.v1i1.29

Issue

Vol. 1 No. 1 (2023): June 2023

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Article

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Copyright (c) 2023 Khairan Khairan, Musfira Arini, Rinaldi Idroes, Khalijah Awang, Claus Jacob

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