Antioxidant Potential of Ethanol Extracts from Zingiberaceae Plant Leaves

Authors

  • Addrian Maulana Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Aufa Sabrina Thahar Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia
  • Khairan Khairan School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia

DOI:

https://doi.org/10.60084/mp.v3i2.337

Keywords:

Ginger leaves, Turmeric leaves, Temulawak leaves , DPPH, ABTS

Abstract

The development of disease related to oxidative stress must be addressed immediately. An approach is to identify natural antioxidant compounds in plants commonly used by communities. This study measured the potential antioxidant activity of ethanol extracts from three Zingiberaceae species leaves: ginger (Zingiber officinale var. Amarum), turmeric (Curcuma domestica Val), and temulawak (Curcuma xanthorrhiza). Phytochemical profiling was performed using specific reagents, FT-IR analysis, and GC-MS, while antioxidant activity was evaluated using DPPH and ABTS methods. The result showed the presence of saponins, flavonoids, steroids, alkaloids, tannins, fatty acid derivatives, and phytol. The IC50 values of the extracts, determined using the DPPH method, were found to be 28.75 ppm for ginger, 65.86 ppm for turmeric, and 51.41 ppm for temulawak. Using the ABTS method, the IC50 values were 35.4 ppm for ginger, 75.9 ppm for turmeric, and 58.9 ppm for temulawak. The strongest antioxidant activity of ethanol leaf extracts from Zingiberaceae family was found in ginger leaf extract with the lowest value of IC50. These results provide preliminary evidence that Zingiberaceae leaves, which are less studied compared to their rhizomes, possess notable antioxidant potential. Further studies, including the isolation of active compounds and in vivo evaluation, are required to validate these findings and explore their possible applications in the future.

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References

  1. Hacke, A. C. M., Litke, Q., Sestric, R., Hardy, B., Kuss, S., Liu, S., Levin, D., and Sorensen, J. L. (2025). Insights into the Antioxidant Activity of Carotenoid Standards, Microalgal, and Yeast Extracts via Spectroscopic and Electrochemical Methods, Food Chemistry, Vol. 495, 146338. doi:10.1016/j.foodchem.2025.146338.
  2. Neha, K., Haider, M. R., Pathak, A., and Yar, M. S. (2019). Medicinal Prospects of Antioxidants: A Review, European Journal of Medicinal Chemistry, Vol. 178, 687–704. doi:10.1016/j.ejmech.2019.06.010.
  3. da Silva Araujo, E., Ferreira, V. R. F., Lorenço, M. S., Zidanes, U. L., da Silva Araujo, E., Campos e Silva, R., de Sousa, I. A. L., da Silva Mota, G., Bufalino, L., das Graças Cardoso, M., de Souza, T. M., and Mori, F. A. (2025). Tannins from Amazonian Tree Barks as Potential Natural Antioxidants: An Alternative to Synthetic Phenolic Compounds, International Journal of Biological Macromolecules, Vol. 323, 147148. doi:10.1016/j.ijbiomac.2025.147148.
  4. Bouayed, J., and Bohn, T. (2010). Exogenous Antioxidants—Double‐Edged Swords in Cellular Redox State: Health Beneficial Effects at Physiologic Doses versus Deleterious Effects at High Doses, Oxidative Medicine and Cellular Longevity, Vol. 3, No. 4, 228–237. doi:10.4161/oxim.3.4.12858.
  5. Sukandiarsyah, F., Purwaningsih, I., and Ratnawaty, G. J. (2023). Aktivitas Antioksidan Ekstrak Rimpang Temu Ireng (Curcuma Aeruginosa Roxb.) Metode DPPH, Jurnal Mandala Pharmacon Indonesia, Vol. 9, No. 1, 62–70. doi:10.35311/jmpi.v9i1.299.
  6. Suprihatin, T., Rahayu, S., Rifa, M., and Widyarti, S. (2020). Compounds in Turmeric Rhizome Powder (Curcuma Longa L.) Which Have Potential as Antioxidants, Buletin Anatomi Dan Fisiologi, Vol. 5, No. 1.
  7. Anukanon, S., Saeng-ngoen, K., Ngamnon, Y., Rapan, N., Seelarat, W., Takolpuckdee, P., Pakvilai, N., and Chatree, Y. (2025). Comparative Analysis of Curcuminoid Content, Antioxidant Capacity, and Target-Specific Molecular Docking of Turmeric Extracts Sourced from Thailand, Food Chemistry: Molecular Sciences, Vol. 11, 100291. doi:10.1016/j.fochms.2025.100291.
  8. Rostamkhani, H., Faghfouri, A. H., Veisi, P., Rahmani, A., Noshadi, N., and Ghoreishi, Z. (2022). The Protective Antioxidant Activity of Ginger Extracts (Zingiber Officinale) in Acute Kidney Injury: A Systematic Review and Meta-Analysis of Animal Studies, Journal of Functional Foods, Vol. 94, 105111. doi:10.1016/j.jff.2022.105111.
  9. Yang, Q., Chen, P., Zhong, R., and Miao, J. (2025). Small Yellow Ginger Extract: Preparation, Characterization, Antioxidant Properties, and Protective Activities against Alcohol-Induced HepG2 Cell Injury, Process Biochemistry, Vol. 157, 183–196. doi:10.1016/j.procbio.2025.07.011.
  10. Sridhar, K., and Charles, A. L. (2019). In Vitro Antioxidant Activity of Kyoho Grape Extracts in DPPH and ABTS Assays: Estimation Methods for EC50 Using Advanced Statistical Programs, Food Chemistry, Vol. 275, 41–49. doi:10.1016/j.foodchem.2018.09.040.
  11. Mendonça, J. da S., Guimarães, R. de C. A., Zorgetto-Pinheiro, V. A., Fernandes, C. D. Pietro, Marcelino, G., Bogo, D., Freitas, K. de C., Hiane, P. A., de Pádua Melo, E. S., Vilela, M. L. B., and Nascimento, V. A. do. (2022). Natural Antioxidant Evaluation: A Review of Detection Methods, Molecules, Vol. 27, No. 11, 3563. doi:10.3390/molecules27113563.
  12. Health, I. M. of. (2017). Indonesian Herbal Pharmacopoeia (Farmakope Herbal Indonesia), 2nd Edition, Indonesian Ministry of Health, Jakarta.
  13. Harborne, J. . (1998). Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th Edition, Chapman and Hall Ltd, London.
  14. Rai, S., Kafle, A., Devkota, H. P., and Bhattarai, A. (2023). Characterization of Saponins from the Leaves and Stem Bark of Jatropha Curcas L. for Surface-Active Properties, Heliyon, Vol. 9, No. 5, e15807. doi:10.1016/j.heliyon.2023.e15807.
  15. Harborne, J. B. (1987). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis.
  16. Lestari, S. M., Camelia, L., Rizki, W. T., Pratama, S., Khutami, C., Amelia, A., Rahmadevi, R., and Andriani, Y. (2024). Hytochemical Analysis and Determination of MIC and MFC of Cacao Leaves Extract (Theobroma Cacao L.) against Malassezia Furfur, Jurnal Jamu Indonesia, Vol. 9, No. 2, 53–66. doi:10.29244/jji.v9i2.316.
  17. Locatelli, M., Gindro, R., Travaglia, F., Coïsson, J.-D., Rinaldi, M., and Arlorio, M. (2009). Study of the DPPH-Scavenging Activity: Development of a Free Software for the Correct Interpretation of Data, Food Chemistry, Vol. 114, No. 3, 889–897. doi:10.1016/j.foodchem.2008.10.035.
  18. Touhamia, Y., Kharrat, N., Aamiri, A., Patel, M., Adnan, M., Rezzoum, N.-E., Pereira, L., Allouche, N., Salah, H. Ben, and Lahcen, T. O. B. (2025). In Vitro and in Silico Assessment of Antioxidant Potential of Crude Sulfated Polysaccharide and Phenolic Extracts from Gracilaria Gracilis Macroalgae Harvested from Atlantic and Mediterranean Coasts: A Comparative Study, Algal Research, Vol. 91, 104229. doi:10.1016/j.algal.2025.104229.
  19. Luo, Y., Li, Y., Shi, C., Min, S., Li, B., Lu, Y., Cao, B., Su, H., and He, Y. (2025). Recent Advances in Flavonoids Benefiting Intestinal Homeostasis, Trends in Food Science & Technology, Vol. 165, 105311. doi:10.1016/j.tifs.2025.105311.
  20. Tuncay, F. O., Cakmak, U., and Kolcuoğlu, Y. (2023). Rhaphiolepis Indica (L.) Lindl. Fruit: LC-HRMS-Based Phytochemical Profile, FTIR Spectral, in Vitro Enzyme Inhibition and Antioxidant Analysis, Food Bioscience, Vol. 56, 103228. doi:10.1016/j.fbio.2023.103228.
  21. Elsadek, M. F., and Al-Numair, K. S. (2024). Profiling of Phytochemical Constituents of Terminalia Chebula Fruit Extract by Different Solvent Effects and Synchronized Analysis of FTIR and GCMS, Journal of King Saud University - Science, Vol. 36, No. 9, 103414. doi:10.1016/j.jksus.2024.103414.
  22. Sadgrove, N., Padilla-González, G., and Phumthum, M. (2022). Fundamental Chemistry of Essential Oils and Volatile Organic Compounds, Methods of Analysis and Authentication, Plants, Vol. 11, No. 6, 789. doi:10.3390/plants11060789.
  23. Fratianni, F., D’Acierno, A., Ombra, M. N., Amato, G., De Feo, V., Ayala-Zavala, J. F., Coppola, R., and Nazzaro, F. (2021). Fatty Acid Composition, Antioxidant, and in Vitro Anti-Inflammatory Activity of Five Cold-Pressed Prunus Seed Oils, and Their Anti-Biofilm Effect Against Pathogenic Bacteria, Frontiers in Nutrition, Vol. 8. doi:10.3389/fnut.2021.775751.
  24. Rosa, G. P., Seca, A. M. L., Pinto, D. C. G. A., and Barreto, M. C. (2024). New Phytol Derivatives with Increased Cosmeceutical Potential, Molecules, Vol. 29, No. 20, 4917. doi:10.3390/molecules29204917.
  25. Wang, H., Wang, Z., Zhang, Z., Liu, J., and Hong, L. (2023). β-Sitosterol as a Promising Anticancer Agent for Chemoprevention and Chemotherapy: Mechanisms of Action and Future Prospects, Advances in Nutrition, Vol. 14, No. 5, 1085–1110. doi:10.1016/j.advnut.2023.05.013.
  26. Molyneux, P. (2004). The Use of the Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity, Songklanakarin J. Sci. Technol, Vol. 26, No. 2, 211–219.

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Published

2025-09-30

How to Cite

Maulana, A., Thahar, A. S., & Khairan, K. (2025). Antioxidant Potential of Ethanol Extracts from Zingiberaceae Plant Leaves. Malacca Pharmaceutics, 3(2), 67–73. https://doi.org/10.60084/mp.v3i2.337

Issue

Vol. 3 No. 2 (2025): September 2025

Section

Article

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Copyright (c) 2025 Addrian Maulana, Aufa Sabrina Thahar, Khairan Khairan

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