In Vitro Test of Antioxidant Activity of Leilem Leaf Ethanol Extract (Clerodendrum minahassae) Using DPPH and FRAP Methods
DOI:
https://doi.org/10.60084/hjas.v2i1.135Keywords:
Clerodendrum minahassae, Free radicals, Phytochemical screening, Antioxidant assayAbstract
The risk of degenerative diseases is increasing due to unhealthy lifestyles. Many people consume junk food, smoke, and do not protect themselves from sun exposure and pollution, exposing their bodies to free radicals. Free radicals can cause oxidative stress, necessitating the need for antioxidants to neutralize them. The leilem plant (Clerodendrum minahassae) is an endemic plant from North Sulawesi that is often used in traditional dishes and medicine. Moreover, leilem leaves are believed to be a source of natural antioxidants. This study aimed to determine the efficacy of leilem leaf ethanol extract as a source of antioxidants. The antioxidant potential was evaluated using phytochemical screening and antioxidant assays such as 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power (FRAP) methods, with ascorbic acid as a comparison. The level of antioxidant activity was measured by determining the IC50 value, which corresponds to the concentration of extract required to inhibit 50% of free radicals. The results of phytochemical screening showed that the ethanol extract of leilem (Clerodendrum minahassae) leaves contains active compounds such as phenols, flavonoids, saponins, tannins, steroids/triterpenoids, and alkaloids, indicating a high potential for antioxidant activity. The IC50 value for the DPPH method was 78.799 ppm, and the IC50 value for the FRAP method was 92.127 ppm. These results demonstrate that the ethanol extract of leilem leaves has strong antioxidant activity in both the DPPH and FRAP methods.
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References
- World Health Organization. (2023). Noncommunicable Diseases, from https://www.who.int/news-room/fact-sheets/detail/noncommunicable-diseases, accessed 20-7-2023.
- Sharifi-Rad, M., Anil Kumar, N. V., Zucca, P., Varoni, E. M., Dini, L., Panzarini, E., Rajkovic, J., Tsouh Fokou, P. V., Azzini, E., Peluso, I., Prakash Mishra, A., Nigam, M., El Rayess, Y., Beyrouthy, M. El, Polito, L., Iriti, M., Martins, N., Martorell, M., Docea, A. O., Setzer, W. N., Calina, D., Cho, W. C., and Sharifi-Rad, J. (2020). Lifestyle, Oxidative Stress, and Antioxidants: Back and Forth in the Pathophysiology of Chronic Diseases, Frontiers in Physiology, Vol. 11. doi:10.3389/fphys.2020.00694.
- Maulana, A., Faisal, F. R., Noviandy, T. R., Rizkia, T., Idroes, G. M., Tallei, T. E., El-Shazly, M., and Idroes, R. (2023). Machine Learning Approach for Diabetes Detection Using Fine-Tuned XGBoost Algorithm, Infolitika Journal of Data Science, Vol. 1, No. 1, 1–7. doi:10.60084/ijds.v1i1.72.
- Martemucci, G., Costagliola, C., Mariano, M., D’andrea, L., Napolitano, P., and D’Alessandro, A. G. (2022). Free Radical Properties, Source and Targets, Antioxidant Consumption and Health, Oxygen, Vol. 2, No. 2, 48–78. doi:10.3390/oxygen2020006.
- Sies, H. (2020). Oxidative Stress: Concept and Some Practical Aspects, Antioxidants, Vol. 9, No. 9, 852. doi:10.3390/antiox9090852.
- Rahaman, M. M., Hossain, R., Herrera‐Bravo, J., Islam, M. T., Atolani, O., Adeyemi, O. S., Owolodun, O. A., Kambizi, L., Daştan, S. D., Calina, D., and Sharifi‐Rad, J. (2023). Natural Antioxidants from Some Fruits, Seeds, Foods, Natural Products, and Associated Health Benefits: An Update, Food Science & Nutrition, Vol. 11, No. 4, 1657–1670. doi:10.1002/fsn3.3217.
- Sari, N. W. S., Tallei, T. E., and Kolondam, B. J. (2023). Benefits of Green Tea Polyphenols for Kidney Health: A Literature Review, Grimsa Journal of Science Engineering and Technology, Vol. 1, No. 2, 60–70. doi:10.61975/gjset.v1i2.13.
- Khairan, K., Arini, M., Idroes, R., Awang, K., and Jacob, C. (2023). Antibacterial Activity of n-Hexane Dragon’s Blood Resin Extract (Daemonorops draco wild Blume) from Bener Meriah, Aceh Province, Indonesia, Malacca Pharmaceutics, Vol. 1, No. 1, 22–29. doi:10.60084/mp.v1i1.29.
- Imelda, E., Fitria, U., Mutia, U. P., Syahrul, S., Sari, M. D., Adev, S. M., Adev, A. M., Zakiaturrahmi, Z., and Toshniwal, N. S. (2023). Hippobroma longiflora L Leaves as a Natural Inhibitor of Cataract Progression: A Comprehensive Study Integrating Ethanol Extract, HPLC, and Molecular Docking Approaches, Grimsa Journal of Science Engineering and Technology, Vol. 1, No. 2, 40–51. doi:10.61975/gjset.v1i2.10.
- Ningsih, D. S., Celik, I., Abas, A. H., Bachtiar, B. M., Kemala, P., Idroes, G. M., and Maulydia, N. B. (2023). Review of the Ethno-dentistry Activities of Calotropis gigantea, Malacca Pharmaceutics, Vol. 1, No. 1, 8–15. doi:10.60084/mp.v1i1.31.
- Dolongtelide, J. I., Fatimawali, F., Tallei, T. E., Suoth, E. J., Simbala, H. E. I., Antasionasti, I., and Kalalo, M. J. (2023). In Vitro Antioxidant Activity of Chrysanthemum indicum Flowers Extract and Its Fraction, Malacca Pharmaceutics, Vol. 1, No. 2, 43–47. doi:10.60084/mp.v1i2.26.
- Christodoulou, M. C., Orellana Palacios, J. C., Hesami, G., Jafarzadeh, S., Lorenzo, J. M., Domínguez, R., Moreno, A., and Hadidi, M. (2022). Spectrophotometric Methods for Measurement of Antioxidant Activity in Food and Pharmaceuticals, Antioxidants, Vol. 11, No. 11, 2213. doi:10.3390/antiox11112213.
- Munteanu, I. G., and Apetrei, C. (2021). Analytical Methods Used in Determining Antioxidant Activity: A Review, International Journal of Molecular Sciences, Vol. 22, No. 7, 3380. doi:10.3390/ijms22073380.
- Rachmatiah, T., Daud, J. J., and Artanti, N. (2022). Aktivitas Antioksidan, Toksisitas, Kandungan Senyawa Fenol dan Flavonoid Total dari Daun Leilem (Clerodendrum minahassae Teijsm. & Binn), Sainstech Farma, Vol. 15, No. 1, 35–43. doi:10.37277/sfj.v15i1.1103.
- Pratiwi, D., and Siampa, J. P. (2023). Analisis Aktivitas Antioksidan Ekstrak dan Fraksi Daun Leilem (Clerodendrum minahassae Teijsm. & Binn.) dengan Metode DPPH (2, 2-difenil-1-pikrihidrazil), Jurnal Lentera Farma, Vol. 2, No. 1, 8–14.
- Kairupan, C. F., Mantiri, F. R., and H Rumende, R. R. (2019). Phytochemical Screening and Antioxidant Activity of Ethanol Extract of Leilem ( Clerodendrum minahassae Teijsm. & Binn) as an Antihyperlipidemic and Antiatherosclerotic Agent, IOP Conference Series: Earth and Environmental Science, Vol. 217, 012016. doi:10.1088/1755-1315/217/1/012016.
- Shaikh, J. R., and Patil, M. (2020). Qualitative Tests for Preliminary Phytochemical Screening: An Overview, International Journal of Chemical Studies, Vol. 8, No. 2, 603–608. doi:10.22271/chemi.2020.v8.i2i.8834.
- Esati, N. K., Jawa La, E. O., and Lestari, G. A. D. (2022). Uji Aktivitas Antioksidan Ekstrak Etanol Daun Rosemary (Rosemarinus officinalis L.) dengan Metode DPPH dan FRAP serta Pengaplikasiannya sebagai Zat Aktif dalam Losion, Jurnal Sains Dan Kesehatan, Vol. 4, No. 4, 363–369. doi:10.25026/jsk.v4i4.1129.
- Gashahun, G. S., and Solomon, L. B. (2018). Study of the Antioxidant Activities of Avocado (Persea americana Mill.) and Three Banana (Musa paradisiac L.) varieties by FRAP and Rancimat Assays, Anatomy Physiology & Biochemistry, Vol. 6, 555678.
- Rizkita, A. D., Maulana, I., and Dewi, S. A. (2023). Detection of Flavonoid Compounds of Daruju Root Extract (Acanthus ilicifolius Linn) using Thin Layer Chromatography and UV-Vis Spectrophotometry, Jurnal Sains Dan Kesehatan, Vol. 5, No. 1, 1–5. doi:10.25026/jsk.v5i1.1185.
- Kepel, B. J., and Bodhi, W. (2020). Standarisasi Parameter Spesifik dan Non-Spesifik Ekstrak Rimpang Lengkuas Merah (Alpinia Purpurata K. Schum) sebagai Obat Antibakteri, EBiomedik, Vol. 8, No. 1.
- Hasan, H., Ain Thomas, N., Hiola, F., Nuzul Ramadhani, F., and Ibrahim, A. S. (2022). Skrining Fitokimia dan Uji Aktivitas Antioksidan Kulit Batang Matoa (Pometia pinnata) Dengan Metode 1,1-Diphenyl-2 picrylhidrazyl (DPPH), Indonesian Journal of Pharmaceutical Education, Vol. 2, No. 1, 67–73. doi:10.37311/ijpe.v2i1.10995.
- Hernández-Rodríguez, P., Baquero, L. P., and Larrota, H. R. (2019). Flavonoids: Potential Therapeutic Agents by Their Antioxidant Capacity, Bioactive Compounds, Elsevier, 265–288. doi:10.1016/B978-0-12-814774-0.00014-1.
- Kumaradewi, D. A. P., Subaidah, W. A., Andayani, Y., and Al-Mokaram, A. (2021). Phytochemical Screening and Activity Test of Antioxidant Ethanol Extract of Buni Leaves (Antidesma bunius L. Spreng) Using DPPH Method, Jurnal Penelitian Pendidikan IPA, Vol. 7, No. 2, 275–280. doi:10.29303/jppipa.v7i2.675.
- Budaraga, I. K., and Putra, D. P. (2021). Analysis Antioxidant IC50 Liquid Smoke of Cocoa Skin with Several Purification Methods, IOP Conference Series: Earth and Environmental Science, Vol. 757, No. 1, 012053. doi:10.1088/1755-1315/757/1/012053.
- Maesaroh, K., Kurnia, D., and Al Anshori, J. (2018). Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin, Chimica et Natura Acta, Vol. 6, No. 2, 93. doi:10.24198/cna.v6.n2.19049.
- Hasanuddin, A. R. P. (2023). Analisis Kadar Antioksidan Pada Ekstrak Daun Binahong Hijau (Anredera cordifolia (Ten.) Steenis), Bioma: Jurnal Biologi Makassar, Vol. 8, No. 2, 66–74.
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